Extracellular vesicles derived miRNAs from bovine milk modulate the inflammatory response to lipopolysaccharide <i>in vitro</i>

نویسندگان

چکیده

Abstract Bovine milk is composed of a variety biologically active compounds including mi-croRNAs (miRNA), which are found within extracellular vesicles (EVs). Extracellular vesicle-derived miRNAs may play crucial role in the regulation immune response. Thus, this study aimed to identify specific present bovine that modulate system’s response infection. To address this, RAW-Blue™ Cells, mouse macrophage reporter cell line was challenged with E. coli lipopolysaccharide (LPS) and incubated pool isolated from through 24-hour period. After incubation, QUANTI-Blue™ assay used detect activation transcription factor NF-κB by different treatments. Raw-Blue™ cells treated induced some extend signaling pathways leading relative control (P&amp;lt;0.01). Bovine-milk derived suppressed LPS-induced activity dose-dependent manner (P=0.01). top expressed driving such effects vitro, real-time qPCR assays were performed samples. The miRNA-profiling revealed presence involved tumor suppression apoptosis. Overall, our preliminary findings indicated have potential LPS vitro. Texas Tech University, School Veterinary Medicine

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ژورنال

عنوان ژورنال: Journal of Immunology

سال: 2023

ISSN: ['1550-6606', '0022-1767']

DOI: https://doi.org/10.4049/jimmunol.210.supp.167.38